ZFIN Image: Iglesias Gonz�lez et al., 2021, FIGURE 1

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Figure Caption

FIGURE 1

Methodology, the transgenic line, and initial clustering. (A) Parts of the spinal cords were collected for zebrafish and mouse. Lateral images depict fluorescent labeling. Tissues were dissociated and scRNA-seq was performed using Smart-Seq2 protocol. (B) Number of cells detected per cell for zebrafish (green used throughout figures) and for mouse dataset (red used throughout figures). (C) UMAP representation of all cells detected for zebrafish. Individual colors are groups detected by leiden clustering. (D) Same as in (C), but for the mouse dataset. (E,F) Heatmap of gene expression for the detected leiden groups. Genes were selected to detect different cell types and lineages. Zebrafish dataset is shown in (E) and mouse dataset is shown in (F). Color represents the mean gene expression in a particular group of cells. Scale bar in (A) equals 100 ?m.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.