ZFIN Image: Huang et al., 2022, Fig. 2

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Fig. 2

RNF12 increases invasion and metastasis of breast cancer cells.

A Transwell migration of control or RNF12 stably depleted MDA-MB-231 cells. Left panel: Representative images of migrated cells. Right panel, fold changes of migrated cell numbers; mean ± SD of triplicates. Scale bar = 50 μm. B Schematic diagram of the zebrafish embryo; shRNF12 or control MDA-MB-231 cells were injected into the Duc of Cuvier of zebrafish embryos at 48 h post fertilization. Representative images of zebrafish at 5 dpi are shown. Percentage of zebrafish embryos exhibiting invasion at 5 dpi. The results of two independent experiments are shown. The yellow arrows indicate invasive cells. C Depletion of RNF12 inhibits lung metastasis of breast cancer in a transplantable nude mouse model. Left panel: representative bioluminescent images of mice injected with control or RNF12-depleted MDA-MB-231 cells into the tail vein 35 days after injection. Right panel: Bioluminescent signal and surface metastases in the lungs were quantified. Scatter plots are shown as mean ± SD. Statistical significance was set at p < 0.05 (*0.01 < p < 0.05; **0.001 < p < 0.01; ***p < 0.001).

Acknowledgments

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