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Fig. 6 Riboflavin supplementation attenuates hepatic steatosis in etfa+/− livers by suppressing mRNA expression of genes in de novo lipogenesis and lipid uptake. Adult zebrafish were exposed to riboflavin, as in Fig. 4. (A) Representative images of ORO staining (n = 5 per group) in the wild type and etfa+/− mutants with or without riboflavin treatment for 1 week; Scale bar, 50 µm. (B) TG analysis in liver lysates from wild-type and etfa+/− livers with or without riboflavin treatment (n = 6 per group). (C) Relative mRNA expression of genes associated with de novo lipogenesis (srebp1, srebp2, fasn, acsl1a, and dgat2) and fatty acid β-oxidation (cpt1a, acads, acadm, acadl, and acadvl) was measured by qRT-PCR (see Materials and Methods; n = 3 per group, *P < 0.05, **P < 0.005).