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FIGURE 6 Proportion of diploid and triploid responders for each startle stimulus per temperature treatment. Proportion of responders for diploid and triploid larvae reared and measured at 23.5°C (A), 26.5°C (B), 29.5°C (C) or reared at 29.5°C and measured at 23.5°C (D). Depicted values are the proportion of responders per trial, where blue points represent diploid larvae and red points represent triploid larvae. Over all the effect of stimulus number was significant (ANOVA, p < 0.001, n = 250) as was the interaction between ploidy level and stimulus number (ANOVA, p < 0.05, n = 250). The effect of ploidy was also analyzed for a subset of each temperature treatment, comparing a model with and without ploidy level included. Ploidy was significant for larvae reared and measured at 23.5°C (ANOVA, p = 0.04, n = 60) and for larvae reared and measured at 26.5°C (ANOVA, p = 0.01, n = 80). Ploidy was not significant for larvae reared and measured at 29.5°C (ANOVA, p = 0.87, n = 50) and for larvae reared at 29.5°C and measured at 23.5°C (ANOVA, p = 0.06, n = 60).