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Fig 4
Expression profiles of globin genes in Clonorchis sinensis adults incubated under various experimental conditions.
Live C. sinensis worms were incubated in the RPMI-1640 medium supplemented with bile, nitrite, or nitrate under 1%, 5%, or 20% oxygen conditions. After 24 h incubations, mRNA extracted from the worms was used in qRT-PCR with primer sets specific to each of the C. sinensis globin genes. Fold increases in the globin gene expression in each of the experimental groups were calculated against the 20% oxygen group without any chemical supplement (n = 2, mean ± SD). ○P < 0.05; ☆P < 0.01; ★P < 0.001.
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