ZFIN Image: Kim et al., 2021, Fig 7

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Figure Caption

Fig 7

Detection of CsMb1 and CsMb3 in the metacercarial stage of Clonorchis sinensis.

The whole-body sections of C. sinensis metacercariae embedded in paraffin were reacted with mouse antibodies specific to the recombinant forms of C. sinensis CsMb1 and CsMb3. Pooled serum from pre-immune mice (n = 3) was used in the control reaction. The positive reactions were developed into a blue color using a blue chromogen substrate for horseradish peroxidase-conjugated to a second antibody. The red arrows mark a follicle-like structure exhibiting a strong positive signal against the anti-CsMb3 antibody in the subtegumental region. EB, excretory bladder; In, intestine; OS, oral sucker; Tg, tegument; VS, ventral sucker. Original magnifications, x 1,000.

Acknowledgments

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