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Fig. 14 Pt suppresses melanogenesis in 72 hpf zebrafish embryos. 72 hpf zebrafish embryos were incubated with vehicle (0.1% DMSO) or Pt (2.5 and 5 μM) or Rv (10 μM) for 24 h (72 + 24 hpf). 1-phenyl-2-thiourea (PTU, 8.8 μM) functioned as a positive control. A stereomicroscope was used to measure the endogenous body pigmentation (A). The method for estimating tyrosinase enzyme activity. (B) and melanin contents (C) was explained in the methodology. Results were calculated from three or more independent experiments. Data were denoted as percentage differences over untreated control zebrafish embryos. Statistical significance was considered as *p < 0.05, **p < 0.01, ***p < 0.001 compared to untreated zebrafish embryos.