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Fig. 13 Pt downregulated the tyrosinase expression and melanin levels in zebrafish. Zebrafish embryos (24 hpf) were incubated with vehicle (0.1% DMSO) or Pt (2.5 and 5 μM) or Rv (10 μM) for 72 hpf. (A) The zebrafish viability was counted under the stereomicroscope. (B) The tyrosinase enzyme activity under different treatment conditions was measured as described in the Methodology Section. (C) Tyrosinase gene expression was measured using the RT-PCR technique. GAPDH functioned as an internal control gene (D) Melanin production induced under different treatment conditions was quantified from zebrafish lysates as explained in the Methodology Section. 1-phenyl-2-thiourea (PTU, 8.8 μM) functioned as a positive control. Results were denoted as mean ± SD of three or more independent experiments. Data were denoted as percentage differences over untreated control zebrafish embryos. Statistical significance was considered as *p < 0.05, **p < 0.01, ***p < 0.001 compared to untreated zebrafish embryos.