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Fig. 8 Pt induced autophagy flux in B16F10 cells. Different concentrations of Pt (0–30 μM, 24, 48, 72 h) were treated to B16F10 cells - (A) MTT assay determined the cell viability. (B–C) The Western blot technique determined the conversion of LC3-I to LC3-II and p62 and ATG4B proteins. The β-actin functioned as a loading control. (D–E) Pt increased AVO formation – B16F10 cells were first treated with 3-MA (1 mM, 1 h) and then with Pt (30 μM, 24 h). A fluorescence microscope (under red filter) was used to visualize the intracellular AVOs (Bar = 50 μm). AVO number is proportional to the intensity of red fluorescence. Values were quantified using Olympus Softimage Solution software. Data were denoted as fold differences over untreated control cells. Results were denoted as mean ± SD of three or more independent experiments. Statistical significance was considered as **p < 0.01, ***p < 0.001 compared to untreated control cells. ###p < 0.001 compared to Pt-alone treated cells.