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Fig. 3 Pt suppressed various melanogenesis-related proteins in B16F10 cells stimulated with α-MSH. (A) B16F10 cells were first treated with Pt (0–30 μM) followed by stimulation or not with α-MSH (1 μM) to determine the expression pattern of different melanogenesis-associated proteins - MC1R (15 min) (A) p-MITF (4 h), MITF (4 h) (B–C), and tyrosinase (24 h), TRP-1 (24 h), and TRP-2 (24 h) (D–E). After incubation, cells were harvested, and protein lysates were put through the Western blot analysis. β-actin functioned as a loading control protein. Results were denoted as mean ± SD of three or more independent experiments. Data were denoted as fold-difference over untreated control. Statistical significance was considered as *p < 0.05, ***p < 0.001 compared to untreated control. #p < 0.05 compared to α-MSH-stimulated cells.