ZFIN Image: Miles et al., 2021, Fig. 6.

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Figure Caption

Fig. 6.

Photoreceptors in pcdh15b mutants develop with defects that progressively worsen with age. (A) Representative TEM images of the central ONL (top row) and OS (bottom row) in wild-type siblings, Δ7 and ins17 mutants at 4 dpf. Green represents deformed/abnormal OSs. Yellow arrowheads point to regions of disconnection between the IS and OS. (B-D) Quantification of the average length of all OSs in the retina per section (B), the proportion of OS morphologies (C) and the percentage of attached/detached OSs (D) in wild-type siblings, Δ7 and ins17 mutants at 4 dpf. The entire ONL was used for quantification. (E,F) Developmental trend of the percentage of abnormal OSs (E) and photoreceptors with detached OSs (F) in the retina from 4 dpf to 10 dpf in wild-type siblings, Δ7 or ins17 mutants. Statistical analyses were performed using Student's t-tests (unpaired, two-tailed) (B-D) and two-way ANOVA (E,F). n.s., not significant; **P≤0.01, ****P≤0.0001. Number of eyes/OSs analysed for 4 dpf data: +/+, 10 eyes/1528 OSs; Δ7/Δ7, 8 eyes/1412 OSs; +/+, 10 eyes/1829 OSs; ins17/ins17, 6 eyes/1328 OSs. IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

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Phenotype details

Acknowledgments

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