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Figure 1.
Dissected brain samples were first subject to staining and tissue clearing. This was followed by whole-mount imaging using light-sheet fluorescence microscopy. Three-dimensional volumes were created from individual image sets, and then registered into the same anatomical space prior to averaging to generate a representative image. Finally, volumes were segmented into over 200 neuroanatomical regions.
Overview of the strategy for generating the adult zebrafish brain atlas (AZBA).