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Figure 5
BTDE decreases the vasculogenic mimicry of A549 cells. (a) A549 was pretreated with BTDE for 24 h, then seeded on matrigel for 30 h, capillary-like structures of A549 were recorded by inverted microscope (original magnification, 4×; scale bar: 600 μm) and total length of tubes was measured by Image J software. ** p < 0.01 versus control. (b) Different concentrations of BTDE were added after tubes were established on matrigel for 6 h, and incubated for another 20 h. Tubular structures were observed by inverted microscope (original magnification, 4×; scale bar: 600 μm) and total length of tubes compared with 0 μM was measured by Image J software. (c) Western blot was used to measure the VEGF, HIF-1α, β-catenin, AKT, and ERK as well as their phosphorylation levels in A549 treated with BTDE for 24 h. Data are represented as mean ± SD of three independent experiments. ** p < 0.01 versus control.