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Figure 4
BTDE decreases the migration of A549, H1975 and the invasion of A549. The effect of BTDE on A549 (a) or H1975 (b) proliferation. A549 or H1975 was incubated with different concentrations of BTDE at 37 °C for 48 h, cell viability was determined by MTT assay. Wound healing of A549 after 36 h (c) or H1975 after 72 h (d) treatment with 0−10 μM BTDE was recorded with inverted microscope (original magnification, 4×; scale bar: 600 μm) and the wound healing area was measured by Image J software. Migration ability of A549 (e) or H1975 (f), invasion ability of A549 (g) treated with 0−10 μM BTDE for 24 h, photos were obtained by inverted microscope (original magnification, 10×; scale bar: 300 μm) and OD values of 570 nm were measured. Data are represented as mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01 versus control.