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Figure 3
BTDE blocks intersegmental vessel formation in zebrafish embryos. (a) Lateral view of Tg (flk1: EGFP) zebrafish embryos at 16 hpf. Embryos were treated with different concentrations of BTDE. Vessels of zebrafish embryos were observed using a fluorescence microscope, photos were recorded by inverted fluorescence microscope (scale bar: 1.2 mm). (b) Quantification of intersegmental vessel growth induced by BTDE. Values represent the means ± SD of three independent experiments. * p < 0.05, ** p < 0.01 versus medium control.
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