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Figure 2
Hyperglycemia does not lead to increased cell death in the neural retina of pdx1−/− mutants. Cryosections of retina at 3 (a,b), 6 (c,d) and 10 (e,f) mpf from control and pdx1−/− mutants labeled by TUNEL staining (green) and counterstained with DAPI (blue). (g) Quantification of TUNEL positive nuclei in all layers of the retina. Box plot extends from 75% to 25%, showing all data points, line indicates median, n = 4–7 fish per time point. Scale bar: 50 µm. (GC, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer).
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