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Figure 10
The effects of 72 h isothiocyanates treatment on morphology and mortality of 48 hpf zebrafish embryos at the experiment starting point. LJ-LNC-2 no effect on vascularization of zebrafish embryos. (A) Representative images of embryos exposed by 1–3, of LJ-NCS-1–5 (n = 3 replicates, 50 embryos per replicate); bar scale = 950 µm. Percentage of viability-data are represented as mean ± SD; ** p < 0.01, *** p < 0.001. (B) Representative picture (taken under fluorescence microscope) of blood vessels (green) of Tg(fli1:EGFP)y1 strain (48 hpf zebrafish embryos at the starting point) upon 3 µM LJ-NCS-2 treatment in 24, 48 and 72 h time points.