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Figure 7
Hair cell recovery at 3 and 4 days post-fertilization (dpf). (A) Boxplots showing the difference in functional (FM+) and (B) total (GFP+) hair cells between the first and second 50 µM neomycin treatments on 3 dpf (I2 minus I1) and 4 dpf (I4 minus I3) in 6- and 12-h treatment timelines. Red dots and text indicate mean values. Statistical analysis was performed using Kruskal–Wallis ANOVA with Dunn post-test. (C) Bar graph showing the difference in the ratio of functional hair cells (FM+) to total hair cells (GFP+) between the first and second treatments on 3 dpf (I2 minus I1) and 4 dpf (I4 minus I3) for 50 µM treatments in 6-h and 12-h treatment timelines. Error bars represent standard deviation, n = 15 for each group. One-tailed z-score tests for proportions were used to evaluate increases in the proportion of FM+ cells. Significance levels are as follows: **** = p < 0.0001, ** = p < 0.01, * = p < 0.05, ns = not significant.