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Fig. 2 Evaluation of prophylactic and therapeutic effect of TnP on neutrophil recruitment. Zebrafish larvae at 72 hpf with amputated tail were exposed to LPS (S. abortus at 10 or 100 μg/mL - A) or TnP alone at 0.0003, 0.003, and 0.01 μM for 1 h (B). TnP at 0.0003, 0.003, and 0.01 μM were applied before (C - prophylactic, TnP/LPS) or after (D - therapeutic, LPS/TnP) LPS stimulation. Larvae with amputated caudal fins non-stimulated and non-treated were considered as control. At the end of experiments, groups of larvae were fixed and stained with Sudan black solution for the absolute neutrophil number count in 500 μm from the cut of the tail. Data represent mean ± SEM and *p < 0.01 compared with control and #p < 0.01 compared with LPS-group.