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Figure 6

ID
ZDB-IMAGE-211120-81
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Figures for Lai et al., 2021
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Figure Caption

Figure 6

Chronic effects of intestinal miR-21 expression on colitis-associated cancer (CAC). (A) Representative images of gross intestinal phenotypes at 7 months post-fertilization (mpf)/2 months post-treatment (mpt) of WT + Dox and ImiR-21 + Dox. Scale bar: 2 mm. (B) H&E-stained intestinal sections of 7 mpf/2 mpt WT + Dox and ImiR-21 + Dox. (α and α’) Intestinal bulb. (β and β’) Mid-intestine. (γ and γ’) Edge of mid-intestine and posterior intestine. Scale bar: 250 μm. (C) RT-qPCR analysis of the mRNA expression of the CAC-associated genes in the intestine of 7 mpf/2 mpt WT + Dox and ImiR-21 + Dox. Gene expression levels were normalized to gapdh levels in WT + Dox. Statistically significant differences from WT + Dox are denoted by * (p < 0.05), ** (p < 0.01), and *** (p < 0.001). Representative images of H&E-stained sections of (D) WT + Dox and (E) ImiR-21 + Dox at 8 mpf/3 mpt. Scale bar: 2 mm.

Acknowledgments
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