FIGURE 3

FIGURE 3

UV and Blue cone photoreceptor morphology paired with gene expression throughout a 28 day lesion and regeneration time-course. (A–H) UV cone photoreceptor degeneration and regeneration is demonstrated in these retinal sections collected at baseline (0 h) through 28 days post phototoxic lesion (dpl), hours post light are denoted (hpl). Sections were immunolabeled with anti-UV Opsin and nuclei were stained blue with TO-PRO-3. Cone photoreceptors are mostly destroyed at 72 hpl after a period of initial hypertrophy (n = 5–6). (I) Graph of transcript pseudo-counts for UV Opsin (opn1sw1) from 3′mRNA-seq of individual adult zebrafish retinas for each timepoint (n = 6). (J) ImageJ pixel intensity quantification for the UV Opsin signal in the confocal images normalized to 1, demonstrating relative intensity of protein localization within the retina. (K) Overlay of opn1sw1 RNA expression normalized to 1 and ImageJ protein localization normalized to one. (L–S) Blue cone photoreceptor degeneration and regeneration. Sections were immunolabeled with anti-Blue Opsin and nuclei were stained blue with TO-PRO-3 (n = 5–6). (T) Graph of transcript pseudo-counts for Blue Opsin (opn1sw2) from 3′mRNA-seq of individual adult zebrafish retinas for each timepoint (n = 6). (U) ImageJ pixel intensity quantification for the Blue Opsin signal in the confocal images normalized to 1, demonstrating relative intensity of protein localization within the retina. (V) Overlay of opn1sw2 RNA expression normalized to 1 and ImageJ protein localization normalized to one. Scale bar represents 5 μm.