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Figure 6
Mannan administration reduces uptake of mA?42 by BLECs and peripheral transport. (A) A schematic diagram of dorsal view of the 3 dpf larval brain and the experimental setup. Dotted gray box denotes the region of interest. (B,C) Confocal images of mrc1a:mCherry+ BLECs co-injected with pHrodoGreen and mA?42 (HiLyte Fluor 647). Arrows indicate colocalization of pHrodoGreen and mA?42 (B). Empty arrowheads show that the mannan administration interferes with colocalization of pHrodoGreen and mA?42. Data are representative of at least three independent experiments. Scale bars = 10 ?m. (D,E) Confocal images of the zebrafish pronephros after mA?42 injection. Dotted lines depict the pronephros structure. Arrowheads indicate the accumulation of mA?42. Mannan treatment prior to mA?42 injection (E) resulted in a reduced pronephric accumulation compared to PBS control (D). Scale bars = 100 ?m. (F) Quantification of the relative ratio of the intensity between the pronephros and brain. Statistical significance was determined by two-tailed unpaired t-test. p = 0.0092. Data are presented as mean ± SEM. n = 9 per group. Data are representative of at least two independent experiments. **, p < 0.01.