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Figure 4
Cell cycle progression and cell proliferation were suppressed in GrnA morphants after PHx. (A) Cell cycle analysis of hepatectomized control and GrnA morphant zebrafish for 0 h (upper) and 24 h (lower) after PHx. PI staining was performed, and samples were analyzed using flow cytometry. The proportion of cells at each stage at 0 h (B) and 24 h (C) after PHx was analyzed by Flow-Jo software and is presented as mean ± SD. The cell cycle regulatory genes included (D) ccnd1, (E) ccne, and (F) ccna2, and (G) myc, the cell proliferation related gene, was probed by quantitative PCR. Data are shown as relative expression of indicated genes normalized to control 0 h after PHx. EF1a served as the internal control. Significance was set at * p < 0.05, ** p < 0.01, *** p < 0.001, as determined by t-test; PHx, partial hepatectomy.