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Figure 6 Oxidative stress in 9-month-old zebrafish livers. (A) NOX4 protein expression (n = 8). (B) Dihydroethidium (DHE) staining of zebrafish livers (n = 3), and (C) quantitation of DHE staining. (D) MDA levels in zebrafish livers (n = 5). *, p < 0.05, **, p < 0.01, ***, p < 0.001. Data represent the mean, and error bars represent SEM. Scale bar, 20 ?m. NAFLD, non-alcoholic fatty liver disease; ND, normal diet; HFD, high fat diet; HEX, high-fat diet plus exercise.
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