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Figure 6 Dopamine-related gene expression in larvae and adult brains. Relative quantification of drd2b (a) and dat1 (b) was measured in 4 dpf larvae and in the brains of developmentally exposed adult fish that were behaviourally tested, using RT-qPCR. β-actin was used as an internal standard. The ΔΔCt method was used to determine the fold change in gene expression. Subject numbers: larvae = 3; adult brains, 0 nM = 13, 5 nM = 6, 10 nM = 5–6, 15 nM = 7–8, 30 nM = 11–12. Significant differences from vehicle control are marked with an asterisk.
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Full text @ Int. J. Mol. Sci.