Fig. 1

Fig. 1

atoh8 (blue) and myoD expression (red) in the gastrula and early segmentation stages of development (6–12 hpf). a Shield stage (6 hpf). b 90% epiboly (9 hpf). b’, b’’ magnifications of image (b) showing dorsal and ventral atoh8 expression, respectively. c 9-hpf embryo double-stained for atoh8 and myoD. datoh8 expression at 9.5 hpf. e, e’ 10-hpf embryos stained for atoh8 alone and atoh8 and myoD, respectively; white arrowhead in e' indicates tail bud tissue free of atoh8 expression. f, f’ 10-hpf embryos stained for atoh8 alone and atoh8 and myoD, respectively. g results of RT-qPCR for atoh8 at developmental stages 2.5 to 11 hpf showing its expression relative to actb2. h, h’ Flat mount and schematic drawing of 10-hpf embryo showing atoh8 and myoD expression in blue and red, respectively. iatoh8 staining at 9.5 hpf. j, j’ 11.5-hpf embryos stained for atoh8 alone and atoh8 and myoD, respectively. k, k’’ 12-hpf embryos stained for atoh8. k’ 12-hpf embryo double-stained for atoh8 and myoD. l, l’ Flat mount and schematic drawing of 12-hpf embryo showing atoh8 and myoD expression in blue and red, respectively. Whole embryos: lateral view, anterior to the top, dorsal to the right. d’ ventral view (v); f, h, k, l dorsal view. Blue and red arrowheads: sites of atoh8 and myoD expression, respectively. Scale bars 200 µm