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FIGURE 4 Relative phyla abundances of microbiome based on 16S rRNA sequencing. (A) Relative abundances of phylum level of two dietary groups (CON and HF). (B) Relative abundances of phylum level between WT and cpt1b?/? zebrafish. (C) Relative abundances of phylum level between WT and pparab?/? zebrafish. (D?F) Ratio of Firmicutes/Bacteroidetes in gut microbiota of (D) dietary groups, (E) WT and cpt1b?/? zebrafish, and (F) WT and pparab?/? zebrafish. (G?I) Ratio of Fusobacteria/Proteobacteria in gut microbiota of (G) dietary groups, (H) WT and cpt1b?/? zebrafish, and (I) WT and pparab?/? zebrafish. All data are normalized to control group (100%) and presented as means ± SEM (**p < 0.01, ***p < 0.001).