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Figure 5
Heteronemin inhibited mitogen-activated protein kinase and transcription factors in human lymphatic endothelial cells. Cells were treated with the indicated concentrations of heteronemin (represented as SP-1); subsequently, the indicated (A) MEK/ERK and (C) p65 phosphorylated and total proteins were determined by Western blot analysis. The quantitative densitometry of phosphorylated (B) MEK/ERK and (D) p65 protein was performed with Image-Pro Plus. Data are expressed as mean ± SEM of five independent experiments. * p < 0.05, compared with the control group.
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