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Figure 3
Heteronemin reduces the VEGF-C-stimulated lymphangiogenesis in mouse ear collagen sponges. Gelatin sponges were saturated with medium containing VEGF-C (1 μg/mL; positive control) with or without indicated concentration of heteronemin (represented as SP-1). Sponges were implanted, for 3 weeks, between the two layers of skin in mice ears. (A) Lymphatic vasculatures in sponge sections were detected by LYVE-1 immunostainings (bars = 100 and 70 μm higher magnification). (B) Quantification of the densities of lymphatic vessels (LYVE-1 positive area). Data are expressed as means ± SEM of five mice. * p < 0.05 versus medium sponges; # p < 0.05 versus VEGF-C-stimulated sponges.