Figure 3

Figure 3 Figure 3. (A) Accumulation of polyubiquitinated proteins and increased in Hsp70 in KG1a and MOLM14 cells exposed to VLX1570; (B) time- and dose-dependent increased in Grp78/BiP in acute myeloid leukemia (AML) cells exposed to VLX1570; thaps: thapsigargin; (C) VLX1570 induction of proteotoxic stress and ER stress; (D) the loss of cell viability is not affected by the pan-caspase inhibitor z-VAD-fmk (10 μM). Cells were exposed to 0.35 μM VLX1570 for 48 h, and cell viability was determined by acridine orange/propidium iodide staining. The barplots are the average of three replicates of a single experiment. Error bars represent one standard deviation. The experiment was repeated with similar results.