FIGURE 3

FIGURE 3 Gata2b is necessary for HSPC specification but dispensable for HSPC maturation. (A–C) Analysis of runx1 expression at 36 hpf by in situ hybridization in Gata2b^u5008 mutant embryos shows decreased expression in gata2b^u5008/u5008 homozygotes. (D) Quantification of runx1 expression in the AGM of 36 hpf mutant embryos shows allele dependent decrease of runx1 expression. (E) Number of itga2b:GFP^lo cells by flow cytometry of individual Tg(itga2b:GFP) Gata2b^u5008 mutant embryos at 3 dpf. (F–H) Expression of c-myb by in situ hybridization in the CHT of 4 dpf mutant larvae. (I) Quantification of c-myb+ cells in the CHT of 4 dpf mutant fish. In (J–L), lateral views of 48 hpf mutant embryos stained with SB, labeling granulocytes. (M) Quantification of SB+ cells in the CHT of 48 hpf mutant embryos. In (N–P), SB stainings of 4 dpf larvae show allele dependent decrease of SB+ cells in the CHT of Gata2b^u5008 mutant fish, quantified in (Q).