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Figure 6
Evaluation of the effect of digoxin and enalapril on the CRS-3 model. (A) Heart images of zebrafish larvae (Control, zebrafish larvae treated with MTZ, zebrafish larvae treated with digoxin or enalapril) (n = 5) at 2 dpi are shown. Scale bar: 100 ?m. (B,C) Heart rate was detected by the dynamic change pattern in the ventricle of zebrafish larvae. (D) ARF-induced heart enlargement can be decreased by digoxin and enalapril (n = 5). (E) Compared with the control group, treatment of ARF larvae with digoxin and enalapril resulted in significant increases in blood flow velocity (n = 5). (F) After treatment with digoxin or enalapril, the survival rate of ARF zebrafish larvae was increased (n = 60). Mean ± SEM; ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001, ANOVA/Dunnett?s test compared with untreated ARF zebrafish larvae.