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Figure 5
Changes of blood flow and blood vessels in ARF larvae. (A) The erythrocytes in the caudal artery (CA) (green arrow), caudal vein (CV) (white arrow), posterior cardinal vein (PCV) (green arrow) and dorsal aorta (DA) (white arrow) of Tg(cdh17:Dendra2-NTR; flk1:GFP;gata1:DsRed) zebrafish larvae treated with MTZ or control are shown. Scale bar: 200 μm. (B) After treatment with MTZ, the caudal artery blood flow velocity of zebrafish larvae (n = 5) was evaluated using ImageJ. (C–E) Confocal images of blood vessels around the heart and tail of Tg(cdh17:Dendra2-NTR; flk1:GFP;gata1:DsRed) zebrafish larvae treated with MTZ or control (n = 5). The Scale bars of (C) and (D,E) are 100 μm and 200 μm, respectively. The red and white line segments represent the vessel lumen of zebrafish larvae. CA, caudal artery; CV, caudal vein. Mean ± SEM; * p < 0.05 and **** p < 0.001, ANOVA/Dunnett’s test compared with control.