ZFIN Image: Peña et al., 2021, FIGURE 4

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Figure Caption

FIGURE 4 Gata2a^um27 and Gata2b^u5008 mutations have independent and non-additive effects on HSPC specification. (A,B) Quantifications of runx1 expression (A) and c-myb+ cells (B) in the AGM of 36 hpf of embryos from Gata2a^+/um27 to Gata2b^+/u5008 crosses. (C–J) Expression of runx1 (C–F) and c-myb (G–J) by in situ hybridization in 36 hpf embryos. In (K,L), mutant fish carrying itga2b:GFP transgene, labeling HSPCs in the CHT, were used to quantify stationary GFP^lo cells in the CHT of 60 hpf (K) and 4 dpf (L) fish. (M) The percentage of embryos displaying blood flow in the trunk in embryos injected with Gata2a (middle), Gata2b (right) mRNA is compared to that of uninjected embryos (left). Absolute numbers of abnormal (pink) and normal (white) embryos are indicated above and under the columns, respectively.

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Acknowledgments

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