Exogenous glucose does not affect neutrophil and macrophage recruitment to a tail wound. (A) Schematic of experiment to measure immune cell recruitment to a tail wound. (B) Representative images of macrophage (red) recruitment to a tail wound in glucose-injected larvae. (C) Quantification of macrophage recruitment following tail transection in the glucose injection model. (D) Quantification of macrophage recruitment following tail transection in the glucose immersion model. (E) Representative images of neutrophil (red) recruitment to a tail wound in glucose-injected larvae. (F) Quantification of neutrophil recruitment following tail transection in the glucose injection model. Each paired data point represents the average of an biological replicate with n > 10 embryos per condition. (G) Quantification of neutrophil recruitment following tail transection in the glucose immersion model. Scale bars represent 100 μm. Statistical testing by t test. Data are representative of 3 biological replicates.
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