ZFIN Image: Lepanto et al., 2021, Fig. 1.

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Figure Caption

Fig. 1.

fabp4a(-2.7):EGFPcaax is expressed in early and mature adipocytes. (A) The upper scheme shows the endogenous fabp4a gene in chromosome 19 with the transcription start site (TSS), exons (boxes), introns, and the coding sequence (CDS) in red. The cloned region is denoted between dashed lines. The lower scheme represents the vector used for transgenesis (tol2: tol2 sites; pA: SV40_late_polyA; cmlc2: cardiac myosin light chain 2 upstream region). (B) Epifluorescence microscopy images of live fabp4a(-2.7):EGFPcaax larvae from the incross of the F3 generation labeled with LipidTOX-Red. EGFP+ cells were present in the PVAT and AVAT depots. Asterisks indicate mature adipocytes stained with LipidTOX-Red with low (*) or high EGFPcaax expression (**). Single arrows denote early adipocytes expressing EGFPcaax with small lipid droplets. Double arrows indicate early adipocytes with EGFPcaax expression without LipidTOX-Red staining. sb, swim bladder; g, gut. Scale bars: B: panoramic views: 200 µm; insets: 50 µm.

Acknowledgments

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