Fig. 2

Fig. 2

Parietopsin acts as the visible light sensor contributing to pineal color discrimination. A Relative difference absorption spectra of parietopsin reconstituted with 11-cis A1 (green, broken curve) and A2 (magenta, solid curve) retinal before and after light irradiation. Parietopsin with A1 retinal is a green light-sensitive pigment (λmax, ~520 nm) similar to previous reports. Reconstitution with A2 retinal shifts the parietopsin spectrum to longer wavelengths (λmax, ~570 nm). B Chromatic response recorded from pineal ganglion cells showing promotion and inhibition of neural firings to visible light (560 nm) and UV light (380 nm), respectively. C The relative response curve of the visible light-sensitive component (promotion of neural firings) in pineal chromatic responses, showing the response peak at 560 nm, consistent with the previous report [4] (n = 6 measurements, see the “Methods” section and Fig. S3 for the details). Error bars indicate SEM. D–F Fluorescence images by immunohistochemical analyses, showing parietopsin (D, green) and Go-type G protein (E, magenta) colocalized in the outer segment (F, merge). Scale bar = 20 μm. G–I Analysis of the photoresponse in a parietopsin-expressing photoreceptor cell by intracellular recording. H is the enlarged image from the white square in G. Note that the location of the pineal slice is shown in Fig. S2D. The cell dye loaded by neurobiotin injection (G and H, magenta) expresses parietopsin (yellow) but not parapinopsin (cyan), showing that the intracellular recording was from a single parietopsin-expressing cell. Note that the dye was loaded into the cell body but not into the parietopsin-containing outer segment because the outer segment consists of stacked membranes and limited cytosolic portion like the case of other photoreceptor cells (see Additional file 2: Movie S1). Scale bar = 100 μm (G), 20 μm (H). Intracellular recording analysis of a parietopsin-expressing photoreceptor cell shows a depolarizing response to visible light (I)