ZFIN Image: Yu et al., 2021, Figure 2

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Figure Caption

Figure 2

Loss of Claudin-5b in zebrafish embryos inhibits activation of autophagy in cerebrovascular endothelial cells (ECs). Morpholino (MO) silencing of claudin-5b in endothelial eGFP-specific transgenic Tg(kdrl:eGFP) zebrafish embryos. (A) Brain phenotype (red arrowheads) of control MO and claudin-5b MO zebrafish larvae in response to hypoxia. Scale bar, 100μm. (B) Quantitative analysis of the severe injury rates of control MO and claudin-5b MO zebrafish larval brains. Mean±SD, n=3 independent experiments per group. (C) Confocal microscopy images of Claudin-5 (red) and LC3 (purple). Scale bar, 5μm. (D) Quantitative analysis of LC3 levels by Image-Pro Plus. Mean±SD, n=3 independent experiments per group. ^*p<0.05 and ^**p<0.01.

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