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Fig. S2 Tg(α-actin::EGFP) embryos that were injected with twist 1a/b, 2, or 3 MO at the one- to four-cell state demonstrated EOM formation at 5 dpf from dorsal (B-D), lateral (B'-D'), Ventral (B''-D''), and phenotype (B‴-D‴) compared with control embryos (A-A‴). SO: Superior Oblique, SR: Superior Rectus, LR: Lateral Rectus, IO: Inferior Oblique, IR: Inferior Rectus. Asterisk: undeveloped jaw muscle, red arrow: incorrectly inserted EOM, scale bar: 100μm.
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