Figure 1

Figure 1

shRNA library screening to identify modulators of cardiomyocyte survival. (a) Schematic overview of the shRNA library screen. HL-1 cells were transduced with a lentiviral shRNA library targeting 4625 genes. Cells were exposed to hypoxia-reoxygenation for a viability dropout screen. As the readout, shRNA abundances were quantified with NGS. (b) Scatter plot illustrating the mean copy number of barcoded shRNAs for each gene after culturing of the cells in normoxia (x-axis) or in hypoxia followed by reoxygenation (y-axis). Significantly regulated genes are depicted with red dots. The dot representing the mean copy number of shRNAs targeting EGFR is indicated in blue. Line depicts the level where shRNA abundances are equal in normoxia and hypoxia-reoxygenation. (c) Volcano plot of log2-transformed fold-changes of mean shRNA counts in normoxic cells and in hypoxia-reoxygenation-treated cells and their respective P values from DESeq2 analysis. Significantly regulated genes are depicted with red dots. The dot representing EGFR is indicated in blue.