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Figure 4
(A, B) Mosaic expression of Ath5 leads to activation of target genes in those cells expressing Ath5. Upper left, DAPI nuclear staining; lower left, HuC fluorescent in situ; upper right, GFP reporter indicating Ath5 expression; and lower right, merge of GFP and in situ signals. (A) Medaka embryo injected with 2-kb Ath5 promoter driving GFP expression in response to Ath5 and Ath5 pCS2+ expression vector. Note the complete overlap of Ath5 activity and expression of its target HuC. (B) Medaka embryo control injected with 2-kb Ath5 promoter driving GFP expression and empty pCS2+ vector.
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