FIGURE 2

FIGURE 2

ACT regulated M1/M2 polarization in LPS-stimulated BV-2 cells. (A) Effects of ACT on LPS-induced BV-2 cell viability. (B) Cell morphology with IBA-1 staining was observed with inverted phase contrast fluorescence microscope (×10). The control group exhibited small soma with distal arborization, showing the typical ramified morphology of resting microglia. The LPS group became fewer and shorter branches with a greatly enlarged cell body, the characteristic shapes of activated microglia. The ACT groups showed attenuated LPS-induced morphological changes. (C) Effects of ACT on LPS-induced elevation of cell supernatant TNF-α, IL-1β, and IL-10 levels as well as NO elevation. *P < 0.05, ***P < 0.005, versus the LPS group. n = 3. Berberine (BBR) as a positive control. (D) Effects of ACT on M1 microglia polarization related markers. (E) Effect of ACT on M2 microglia polarization related markers. *P < 0.05, **P < 0.01, ***P < 0.005, versus the LPS group. n = 3.