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Figure 6 Determination of (A) GABA, (B) GLU, (C) GLU/GABA ratio, and (D) the total ion chromatogram (A) of the water: acetonitrile extract from zebrafish larvae with extracted ion chromatograms and MS/MS spectra of GLU and GABA in 7-day-old zebrafish larvae by HPLC-MS. After a 24 h incubation in 6-GIN (37.5 µM), zebrafish larvae were exposed to an acute dose of PTZ (20 mM) for 90 min. Next, whole zebrafish larvae were collected in a pool of n = 100/sample. Data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. Data are depicted as mean ± SEM. Veh + veh (n = 9), 6-GIN 37.5 + Veh (n = 5), Veh + PTZ (n = 5), 6-GIN 37.5 + PTZ (n = 5). *** p < 0.001, ** p < 0.01, * p < 0.05 vs. Veh + veh; &&& p < 0.001 vs. Veh + PTZ. 6-GIN—6-gingerol, PTZ—pentylenetetrazole, Veh—vehicle. n—refers to the total number of samples.