IMAGE

Fig 1

ID

ZDB-IMAGE-210801-44

Genes

igf2bp3

Publication

Vong et al., 2021 - The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish

All Figures

Figures for Vong et al., 2021

Image

Figure Caption

Fig 1 Loss of <italic toggle='yes'>igf2bp3</italic> results in skewed male:female sex ratios.

A. Generation of the igf2bp3 mutant allele by Cas9. Schematic showing the igf2bp3 locus on chromosome 19. Exon 1 of igf2bp3 was targeted by CRISPR-Cas9, resulting in a 7 bp deletion and frameshift. B. Schematic of the igf2bp3^la659Tg with a 6 kb Tg(nLacZ-GTvirus) retroviral insertion in intron 1. C. The igf2bp3^Δ7 mutant is predicted to be protein null. The igf2bp3^Δ7 mutation generates a premature stop codon at residue 46 whereas the igf2bp3^la659Tg results in a predicted truncated protein of 65 amino acids. D. Loss of igf2bp3 results in skewed sex ratios. Adult zygotic igf2bp3^Δ7 and MZigf2bp3^la659Tg mutant fish show a significant male bias compared to wild type, heterozygous igf2bp3^Δ7 as well as igf2bp3^la659Tg, and Zigf2bp3^la659Tg fish. Asterisks indicate level of significance (p *<0.05, **<0.01, ***<0.001). E. The igf2bp3^Δ7 mutation results in reduced igf2bp3 levels. qRT-PCRs at the 1-cell and 4-cell stages show significantly reduced igf2bp3 transcript levels in igf2bp3^Δ7 mutant embryos (p<0.001).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.