ZFIN Image: Wang et al., 2021, Fig. 2

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Figure Caption

Fig. 2

Frog prestin localized in the cell membrane. A Donor vector (upper one) designed for expression of ranaprestin-EGFP fusion protein and CRISPR/Cas9-mediated gene editing vector (lower one). B Membrane expression of rana prestin (rPres) in HEK293T cells was examined using confocal microscopy. The left one was a detached cell and the right one was an attached cell. C Predicted protein structure of rana prestin (left). The transmembrane domain holds two intertwined inverted repeats of seven TM segments. The N- and C-terminal halves of the TM domain are green and pink, respectively. The STAS domain is within the purple part. Predicted protein structure of gerbil prestin (right)

Acknowledgments

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