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Fig. 8 HKi2 induces expression of klf2a in arterial and venous endothelium in zebrafish. (A,B) Tg(klf2a:H2b-EGFP;kdrl:mCherry) zebrafish embryos klf2a expression reporter (EGFP) and endothelial cells labeling (mCherry) after treatment with: (A) 4 μM HKi2; or (B) 4 μM inactive compound, 2-hydroxy-1-naphthoic acid (compound 9). The compounds were added at 26 hpf, and images were taken at 30 hpf. The trunk vessels were scanned using Airyscan. Star and square indicate dorsal aorta and posterior cardinal vein, respectively. Lateral view with anterior to the bottom and dorsal to the top. (C) Quantification of the EGFP fluorescence intensity using imageJ. A total of seven nuclei were analyzed per embryo, two HKi2 treated and two inactive compound embryos per experiment, and results from two independent experiments were analyzed. (n = 2, one-way ANOVA). **, p < 0.01; ***, p < 0.001.