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Figure 2 The effect of Gyp treatment on cone degeneration. (A) Immunostaining of retinal sections of wildtype, rpgrip1 mutant and Gyp-treated rpgrip1 mutant zebrafish at 6 mpf using zpr-1 antibody where zpr1 stained the cones in green and DAPI labelled nuclei in blue. (B) Intensity fluorescence signals (the corrected total cell fluorescence, CTCF) were measured using ImageJ. Statistical comparisons between individual groups were carried out using one-way ANOVA followed by Bonferroni’s test. Data are displayed as mean ± SEM (n = 5 animals of each group). Ns, no significance; * p < 0.05, *** p < 0.001. INL, inner nuclear layer; ONL, outer nuclear layer.