Figure 2.

Figure 2.

SIRT3 is required to control pathological inflammation and mitochondrial damage during Mabc-R infection. (a and b) SIRT3 WT and KO mice (n = 8 each group) were infected intranasally with Mabc-R (1 × 10⁷ CFU) and monitored at 1 and 3 dpi. (a) Lung tissues were subjected to quantitative real-time PCR analysis for the measurement of mRNA expression of various cytokines/chemokines. (b) The supernatants from lung lysates were subjected to ELISA analysis of TNF (at 3 dpi). (c) SIRT3 WT and KO mice (n = 3 each group) were infected intranasally with Mabc-R (1 × 10⁷ CFU) and monitored at 5 dpi. The lung tissues were harvested and then subjected to TEM analysis (left). Mitochondria with complete cristae are shown in a; swollen mitochondria with vacuolation in the cristae are shown in b. Right, Quantitative analysis of at least 8 EM images in the lung tissues from SIRT3 WT and KO mice infected intranasally with Mabc-R (1 × 10⁷ CFU; n = 3 each group). The ratio of damaged mitochondria in total mitochondria was calculated quantitatively. Scale bars, 500 nm. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant compared with SIRT3 WT conditions (a). Non-parametric test (a, b, and c right). Data represent three independent experiments (c left), and values represent means (± SEM) from three or four independent experiments performed in triplicate (a and b).