Figure 2—figure supplement 3.

Figure 2—figure supplement 3.

(A) Measurement of Squint-sfGFP gradients generated by transplanted source cells. Donor embryos were injected with 250 pg sqt-sfgfp mRNA and Alexa-647 dextran at the one-cell stage. At sphere stage, cells from the animal pole of donor embryos were transplanted to the animal pole of wild-type (top), MZoep (middle), or MZoep embryos injected with an excess of oep mRNA at the one-cell stage. Gradients were visualized in grafted embryos by confocal microscopy 100 min after transplantation. Images are maximum intensity projections of 15 consecutive confocal slices. White contours mark source boundaries as determined by segmentation of Alexa-647 channel images. (B) Quantification of Squint-sfGFP gradients created by ectopic sources. Average GFP intensities at each position were compiled from maximum intensity projections and normalized to the intensity adjacent to the source. Gradients from wild-type (blue), MZoep (red) and oep-injected MZoep (green) embryos were compiled from 9, 8, and 11 grafted embryos, respectively. Error bars denote the standard error of the mean at each position. (C) Exponential fits for gradients in wild-type (blue), MZoep (red), and oep-injected MZoep (green) hosts are plotted. Dashed contours indicate 95% confidence intervals for each exponential fit. Experimental data for each condition are scattered as points in the corresponding color. (D) Comparison of exponential fit parameters for wild-type (blue), MZoep (red), and oep-injected MZoep (green) host embryos. Error bars denote 95% confidence intervals.