ZFIN Image: Butti et al., 2021, Fig. 7

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Figure Caption

Fig. 7 Sv2a interacts with C9orf72 and its protein expression is downregulated in C9-miR fish.

a Volcano plot showing the log2 fold change against the −log10 p value. Proteins in blue are upregulated while proteins in red are downregulated (log2 fold change of −1.5 and 1.5, delineated by vertical lines) and are significantly dysregulated (−log10 (p value) > 1.301, delineated by horizontal dotted line) between control and C9-miR fish (N = 4). b Differentially expressed proteins with p < 0.05 in C9-miR 6 dpf larvae (N = 4). c Immunoblot analysis of RFP-immunoprecipitated proteins and lysate of HEK293T cells co-expressing RFP-tagged C9orf72 or RFP in combination with Myc-tagged SV2a or Myc-tagged SMRC8 (a known interactor of C9orf72; positive control). Myc-SV2a and Myc-SMCR8 were extracted out of solution in samples where RFP-C9orf72 was co-transfected, but not in samples where RFP was co-transfected (N = 3).

Acknowledgments

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